antimouse tm4sf1  (R&D Systems)

Journal: Nature Communications

Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression

doi: 10.1038/s41467-025-59888-8

Figure Lengend Snippet: A Volcano plot comparison of all UC and HV cells after downsampling ( N = 150 cells per patient) ( p -values are computed from the two-sided Wilcoxon rank-sum test). B Violin plots of TM4SF1 expression by tumor cluster. Source data are provided as a Source data file. C , D Immunohistochemistry of TM4SF1 in a validation cohort of HV and UC C primary tumors and D lymph node metastases. E Semiquantitative comparison of TM4SF1 staining in HV and UCs (Fisher’s exact test, p = 0.02). Scale bars = 50 µm.

Article Snippet: Flow cytometric quantification of TM4SF1 expression across human bladder cancer cell lines was performed by incubating with anti-TM4SF1-PE antibody (Miltenyi, clone REA851, 1:100) for 30–60 min on ice.

Techniques: Comparison, Expressing, Immunohistochemistry, Biomarker Discovery, Staining

Journal: Nature Communications

Article Title: Bladder cancer variants share aggressive features including a CA125+ cell state and targetable TM4SF1 expression

doi: 10.1038/s41467-025-59888-8

Figure Lengend Snippet: A Quantification of in vitro TM4SF1-CAR1 and CAR2 activity against bladder cancer cell lines using IncuCyte co-culture assay with 1:1 effector:target cell ratio. B In vitro co-culture assays comparing CAR activity (2:1 effector:target cell ratio) in UMUC3 cell lines with and without CRISPR-mediated TM4SF1 knockout ( N = 1 replicate per condition, 5 conditions per experiment). C Evaluation of CAR1 tumor-killing efficacy in an in vivo xenograft model using UMUC3 ( N = 9 mice) and 253JBV ( N = 10 mice) cell lines. Data are presented as mean values ± standard error bars. D Kaplan–Meier survival analysis of CAR1 treated and untreated mice with UMUC3 ( N = 9 mice) and 253JBV ( N = 10 mice) tumors. E:T Effector:Target, UTD untransduced, CAR chimeric antigen receptor.

Article Snippet: Flow cytometric quantification of TM4SF1 expression across human bladder cancer cell lines was performed by incubating with anti-TM4SF1-PE antibody (Miltenyi, clone REA851, 1:100) for 30–60 min on ice.

Techniques: In Vitro, Activity Assay, Co-culture Assay, Co-Culture Assay, CRISPR, Knock-Out, In Vivo